The League says patients and staff at the Horton General Hospital, Banbury will benefit from the cash donation by Castle Lodge, based in Banbury.
The money will be used for equipment to help staff and patient care at the Banbury hospital.
Heather Clelford, Chair of Friends of the Horton, which runs the Horton League of Friends, said: “We are so thankful for this generous donation from Castle Lodge.
Mark Adams, Charity Steward at Castle Lodge in Banbury, said: “Every year we elect to support several local charities and are pleased to donate this money to the Horton League of Friends.
“The League provides valuable support for our community and we hope the donation makes a real difference.”